HIV-1 Coreceptor Tropism, Proviral DNA
Order Code:
HIV1CO
Test Summary
The use of CCR5 antagonists requires screening for viral tropism to exclude patients harboring X4 or D/M virus. HIV-1 Proviral DNA tropism test is useful for determination of viral tropism in patients with undetectable HIV-1 viral RNA (virologically suppressed).
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
2 mL whole blood collected in a K2 EDTA (lavender-top) plastic tube
Minimum Volume
0.6 mL
Instructions
Whole blood: Collect blood in a K2 EDTA (lavender-top) tube.
Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab.
Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab.
Patient Preparation
N/A
Storage
K2 EDTA (lavender-top) plastic tube
Transport Temperature
Whole blood: Room temperature
Peripheral blood: Frozen
Peripheral blood: Frozen
Specimen Stability
- Whole blood
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: 28 days
- Peripheral blood mononuclear cells
- Room temperature: See Collection Instructions
- Refrigerated: See Collection Instructions
- Frozen -20° C: See Collection Instructions
- Frozen -70° C: 28 days
Limitations
N/A
Other Acceptable Specimens
3 mL peripheral blood mononuclear cells
Unacceptable Specimens
N/A
Order Code
HIV1CO
EPIC (Premier) Code
LAB5351
Includes
CXCR4(X4)
Net Tropism Assessment
MVC Activity Anticipated
Net Tropism Assessment
MVC Activity Anticipated
CPT Code
- 87906
Billing Code
- 670911
CPT Statement
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Monday, Thursday; Report available: 4 - 8 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
2 mL whole blood collected in a K2 EDTA (lavender-top) plastic tube
Minimum Volume
0.6 mL
Instructions
Whole blood: Collect blood in a K2 EDTA (lavender-top) tube.
Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab.
Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab.
Patient Preparation
N/A
Storage
K2 EDTA (lavender-top) plastic tube
Transport Temperature
Whole blood: Room temperature
Peripheral blood: Frozen
Peripheral blood: Frozen
Specimen Stability
- Whole blood
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: 28 days
- Peripheral blood mononuclear cells
- Room temperature: See Collection Instructions
- Refrigerated: See Collection Instructions
- Frozen -20° C: See Collection Instructions
- Frozen -70° C: 28 days
Limitations
N/A
Other Acceptable Specimens
3 mL peripheral blood mononuclear cells
Unacceptable Specimens
N/A
Result Information
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Monday, Thursday; Report available: 4 - 8 days