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Estrogens, Fractionated, LC/MS

Test Summary

This panel measures the concentrations of estrone, estradiol, and estriol, the 3 main types of estrogens in serum. This panel may be useful in assessing estrogen status.

Estrogens are endogenous steroids that regulate growth and maintenance of sex organs and secondary sex characteristics in women. Estrogens also have effects on many other organ systems such as maintenance of bone density, production of liver proteins, arterial vasodilation, reduction of intraocular pressure, and influence on mood [1]. Measurements of estrogens may help evaluating sexual maturity, menstrual abnormalities, fertility abnormalities, fetal-placental health in pregnancy, tumors that excrete estrogens, and feminization syndromes in men [2].

Increased levels of estrogens may be observed in normal pregnancy, precocious puberty, hyperthyroidism, liver cirrhosis, and ovarian, testicular, and adrenal tumors. Decreased levels of estrogens may be observed in failing pregnancy, Turner syndrome, hypopituitarism, primary and secondary hypogonadism, menopause, Stein-Leventhal syndrome, and anorexia nervosa [3].

Estriol becomes the predominant estrogen only in late pregnancy. Measurements of estriol are commonly used to help screen for certain fetal abnormalities such as Down syndrome. In non-pregnant women and men, measurements of estriol have limited clinical use [2].

The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.

References
1. Gruber CJ, et al. N Engl J Med. 2002;346(5):340-352.
2. Haymond S, et al. Reproductive related disorders. In: Burtis CA, et al. eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th ed. Elsevier Inc; 2006:2097-2152.
3. Pagana K, et al. Mosby's Diagnostic and Laboratory Test Reference. 15th ed. Elsevier Inc; 2020:346-400.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

2.5 mL Serum in Red Top tube

Minimum Volume

1.5 mL

Instructions

Specify age and sex on test request form

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Refrigerated

Specimen Stability

  • Room temperature: 24 hours
  • Refrigerated: 7 days
  • Frozen: 28 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Received room temperature
Received in an SST
Gross hemolysis, grossly icteric, lipemia

Order Code

ESTRGF

EPIC (Premier) Code

LAB3868

Includes

Estrone
Estradiol, Ultrasensitive, LC/MS
Estriol, Serum

CPT Code

  • 82671

Billing Code

  • 670402

CPT Statement

Methodology

Chromatography/Mass Spectrometry

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675

Department

Reference Testing

Reference Range

See individual tests

Setup Schedule / Expected Turnaround Time

Daily; Report available: 2 - 5 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

2.5 mL Serum in Red Top tube

Minimum Volume

1.5 mL

Instructions

Specify age and sex on test request form

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Refrigerated

Specimen Stability

  • Room temperature: 24 hours
  • Refrigerated: 7 days
  • Frozen: 28 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Received room temperature
Received in an SST
Gross hemolysis, grossly icteric, lipemia

Billing

CPT Code

  • 82671

Billing Code

  • 670402

CPT Statement

Result Information

Methodology

Chromatography/Mass Spectrometry

Testing Laboratory

N/A

Reference Range

See individual tests

Setup Schedule / Expected Turnaround Time

Daily; Report available: 2 - 5 days