Eosinophil Cationic Protein (ECP)
Order Code:
ECP
Test Summary
UNICAP ECP is an invitro test system for quantitative measurement of Eosinophil Cationic Protein (ECP) in human serum. Determination of ECP levels in human sera is important to diagnose a variety of allergic disorders and other clinic conditions connected with activated eosinophils.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.3 mL
Instructions
Parameters such as blood collection tube, coagulation time and temperature must be kept within specified limits, since they affect the concentration of released ECP in serum samples. The clotting represents the first incubation in the assay, in which the ECP measured is to be reproducibly released from the eosinophils which have been activated by the inflammation.
Collect blood by venipuncture. After collection gently invert the tube 5 times. Release ECP by clotting for 60-120 minutes at room temperature 20-24° C. The temperature must not vary more than + or - 1 degree between sampling occasions to give comparable results. Centrifuge at 1000-1300 x g for 10 minutes at room temperature. Decant serum into a new tube.
Collect blood by venipuncture. After collection gently invert the tube 5 times. Release ECP by clotting for 60-120 minutes at room temperature 20-24° C. The temperature must not vary more than + or - 1 degree between sampling occasions to give comparable results. Centrifuge at 1000-1300 x g for 10 minutes at room temperature. Decant serum into a new tube.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 24 hours
- Refrigerated: 7 days
- Frozen: 30 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis • Grossly lipemic • Serum Separator Tube (SST®)
Order Code
ECP
EPIC (Premier) Code
LAB6332
Includes
N/A
CPT Code
- 83520
Billing Code
- 670994
CPT Statement
Methodology
Fluoroenzyme Immunoassay (UNICAP)
FDA Status
This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
2-10 mcg/L
Setup Schedule / Expected Turnaround Time
Tuesday, Friday morning; Report available: 5 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.3 mL
Instructions
Parameters such as blood collection tube, coagulation time and temperature must be kept within specified limits, since they affect the concentration of released ECP in serum samples. The clotting represents the first incubation in the assay, in which the ECP measured is to be reproducibly released from the eosinophils which have been activated by the inflammation.
Collect blood by venipuncture. After collection gently invert the tube 5 times. Release ECP by clotting for 60-120 minutes at room temperature 20-24° C. The temperature must not vary more than + or - 1 degree between sampling occasions to give comparable results. Centrifuge at 1000-1300 x g for 10 minutes at room temperature. Decant serum into a new tube.
Collect blood by venipuncture. After collection gently invert the tube 5 times. Release ECP by clotting for 60-120 minutes at room temperature 20-24° C. The temperature must not vary more than + or - 1 degree between sampling occasions to give comparable results. Centrifuge at 1000-1300 x g for 10 minutes at room temperature. Decant serum into a new tube.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 24 hours
- Refrigerated: 7 days
- Frozen: 30 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis • Grossly lipemic • Serum Separator Tube (SST®)
Result Information
Methodology
Fluoroenzyme Immunoassay (UNICAP)
Testing Laboratory
N/A
Reference Range
2-10 mcg/L
Setup Schedule / Expected Turnaround Time
Tuesday, Friday morning; Report available: 5 days