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Test Directory

Test Directory Cortisol, Total, LC/MS

Cortisol, Total, LC/MS

Test Summary

Total cortisol concentrations are decreased in Addison's Disease and increased in Cushing's Disease and in other conditions of glucocorticoid excess.

Aliases

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum collected in a red-top tube (no gel)

Minimum Volume

0.2 mL

Instructions

Specify time of day specimen was collected

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 14 days
Refrigerated: 21 days
Frozen: 1 year

Limitations

N/A

Other Acceptable Specimens

Plasma collected in: EDTA (lavender-top) tube

Unacceptable Specimens

Gross hemolysis • Serum separator tube (SST)

Order Code

CORTIS

EPIC (Premier) Code

LAB5168

Includes

N/A

CPT Code

82533

Billing Code

670394

CPT Statement

Methodology

Chromatography/Mass Spectrometry

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675

Department

Reference Testing

Reference Range

Adult
8:00 - 10:00 AM	4.6-20.6 mcg/dL
4:00 - 6:00 PM	1.8-13.6 mcg/dL

Cortisol Response to ACTH
Peak	>20.0 mcg/dL
Peak	>16.0 mcg/dL after IM injection

Pediatric

Age	mcg/dL
Infants
Premature (31-35 weeks)**	≤15.0
Term, 3 days old**	≤14.0
<30 days	Not established
1-11 months	1.4-24.0
1 year	1.7-25.4
2 years	1.8-26.4
3 years	2.0-27.0
4 years	2.0-27.2
5 years	2.0-27.3
6 years	2.0-27.2
7 years	2.0-27.1
8 years	2.0-27.0
9 years	1.9-26.8
10 years	1.9-26.4
11 years	1.8-26.1
12 years	1.8-26.0
13 years	1.8-26.1
14 years	1.9-26.6
15 years	2.1-27.5
16 years	2.3-28.6
17 years	2.6-29.8

Pediatric lower reference values are reflective of both AM and early PM sampling
⁠⁠⁠⁠⁠⁠⁠

ACTH Stimulation**	Baseline (mcg/dL)	60 Minutes (mcg/dL)
1-12 Months	3.0-23.0	32.0-60.0
1-5 Years	6.0-25.0	22.0-40.0
6-12 Years	3.0-15.0	17.0-28.0

**Pediatric data from J Clin Endocrinol Metab
(1991) 73:674-686 and J Clin Endocrinol Metab
(1989) 69:1133-1136.

Setup Schedule / Expected Turnaround Time

Monday - Friday; Report available: 3 - 7 days