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Test Directory

C4d Fragment, EIA

Test Summary

Clinical use is to exclude known rheumatoid arthritis, systemic lupus erythematosus, and chronic urticaria with hypocomplementemia. C4d is a stable activation product of C4 cleavage, and is useful to determine classical pathway complement activation.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL frozen plasma collected in an EDTA (lavender-top) tube

Minimum Volume

0.2 mL

Instructions

Barrier tubes (serum separator tubes or plasma separator tubes) are preferred. If non-barrier tubes are utilized, the serum/plasma must be removed immediately after centrifuging and placed in a screw-cap transfer tube. Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered. Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Frozen

Specimen Stability

  • Room temperature: Unacceptable
  • Refrigerated: 4 hours
  • Frozen: 14 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Hemolysis • Serum • Gel barrier interferes with assay methodology

Order Code

C4DF

EPIC (Premier) Code

LAB3547

Includes

N/A

CPT Code

  • 83520

Billing Code

  • 670988

CPT Statement

Methodology

Enzyme Immunoassay (EIA)

FDA Status

This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675

Department

Reference Testing

Reference Range

0.9-8.0 mcg/mL

Setup Schedule / Expected Turnaround Time

Tuesday night; Report available: 4 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL frozen plasma collected in an EDTA (lavender-top) tube

Minimum Volume

0.2 mL

Instructions

Barrier tubes (serum separator tubes or plasma separator tubes) are preferred. If non-barrier tubes are utilized, the serum/plasma must be removed immediately after centrifuging and placed in a screw-cap transfer tube. Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered. Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Frozen

Specimen Stability

  • Room temperature: Unacceptable
  • Refrigerated: 4 hours
  • Frozen: 14 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Hemolysis • Serum • Gel barrier interferes with assay methodology

Billing

CPT Code

  • 83520

Billing Code

  • 670988

CPT Statement

Result Information

Methodology

Enzyme Immunoassay (EIA)

Testing Laboratory

N/A

Reference Range

0.9-8.0 mcg/mL

Setup Schedule / Expected Turnaround Time

Tuesday night; Report available: 4 days