Epidermal Antibodies with Reflex to Titers
Test Summary
The detection of anti-skin antibodies aids in the diagnosis of chronic vesicular-bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA).
Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of sera from all active forms. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.5 mL serum
Minimum Volume
0.1 mL
Instructions
N/A
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen -20° C: 30 days
- Frozen -70° C: Indefinitely
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Gross hemolysis • Grossly lipemic • Grossly icteric
Order Code
AEPIAB
EPIC (Premier) Code
LAB3873
Includes
Intercellular and Basement Antibody Screens with Reflex to Titers
If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).
If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).
CPT Code
- 86255 (x2)
Billing Code
- 670524
CPT Statement
Methodology
Immunofluorescence Assay (IFA)
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
| Intercellular Substance Ab | Negative |
| Titer | <1:10 |
| Basement Membrane Zone Ab | Negative |
| Titer | <1:10 |
Setup Schedule / Expected Turnaround Time
Wednesday, Friday morning; Report available: 4 - 9 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.5 mL serum
Minimum Volume
0.1 mL
Instructions
N/A
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen -20° C: 30 days
- Frozen -70° C: Indefinitely
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Gross hemolysis • Grossly lipemic • Grossly icteric
Result Information
Methodology
Immunofluorescence Assay (IFA)
Testing Laboratory
N/A
Reference Range
| Intercellular Substance Ab | Negative |
| Titer | <1:10 |
| Basement Membrane Zone Ab | Negative |
| Titer | <1:10 |
Setup Schedule / Expected Turnaround Time
Wednesday, Friday morning; Report available: 4 - 9 days